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Myeloperoxidase Human ELISA Kit

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Referência K060-H1

Tamanho : 1x96wellplate

Marca : Arbor Assays

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Myeloperoxidase (MPO) Human ELISA Kit

The DetectX-H.pdf?ver=1738266540"> Product Protocol

Specifications

  • Catalog Number K060-H1
  • Assay Type Sandwich ELISA
  • Sample Types Serum, Platelet-Poor Heparin Plasma, Saliva, Urine, Tissue Culture Media
  • Sensitivity 68 pg/mL
  • Species Human
  • Assay Duration 2.5 Hours
  • Samples/Plate 40 in duplicate
  • Readout Colorimetric, 450 nm

Images

  • Myeloperoxidase (MPO) Human ELISA Kit

    MPO Human ELISA Kit - Typical Standard Curve

Recent Publications

View All 1 Publications

  • Effect of myeloperoxidase on the anticoagulant activity of low molecular weight heparin and rivaroxaban in an in vitro tumor model

    Published in 2020
    Species: Human |
    Sample: Plasma |

    Featured Product

    Myeloperoxidase (MPO) Human ELISA Kit

Assay Principle:>

The DetectX hours. Please read the complete kit insert for more information before performing this assay. Use our provided MPO standard to generate a standard curve for the assay.

Protocol Summary:

  • Introduce standards or diluted samples into the included transparent microtiter plate, pre-coated with anti-human MPO monoclonal antibody.
  • Incubate the plate covered, shaking for 1 hour at room temperature.
  • Post-incubation, wash the plate and add the peroxidase-conjugated human MPO antibody.
  • Incubate again, covered and shaking, for 1 hour at room temperature. The immunological reaction inversely correlates with the MPO concentration in the sample.
  • Wash away excess conjugate and add the TMB substrate, which reacts with the bound antibody to produce a detectable signal.
  • Measure the signal at 450nm using a plate reader and calculate MPO concentration based on the standard curve.

Background:

Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes, where it plays a critical anti-microbial role. Stored in azurophilic granules, MPO is released during neutrophil degranulation and contributes to the formation of hypochlorous acid from hydrogen peroxide and chloride ions in the ‘respiratory burst.’ It also catalyzes the oxidation of tyrosine to tyrosyl radicals, both of which are cytotoxic agents against bacteria and other pathogens. A hereditary MPO deficiency can lead to increased susceptibility to infections.

MPO levels have significant clinical implications, especially in cardiology. Numerous studies have established a correlation between elevated MPO levels and coronary artery diseases, positioning MPO as a potential predictor for myocardial infarction in patients with chest pain. The combination of MPO and C-reactive protein (CRP) measurements has been found to provide a more accurate assessment of mortality risk than CRP alone, highlighting the relevance of MPO in diagnostic and prognostic healthcare settings.

The DetectX;s role in various pathological and physiological processes, particularly in immunology and cardiovascular research.

 

Myeloperoxidase (MPO) Human ELISA Kit

1 Plate K060-H1
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Research Areas

  • Inflammation
  • Oxidative Stress
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