Natriuretic Peptide Receptor C (NPR3) (NM_000908) Human Tagged ORF Clone
Referência RC219453
Tamanho : 10ug
Contactar o distribuidor local :
| Product Data | |
| Target Symbol | Natriuretic Peptide Receptor C |
|---|---|
| Synonyms | ANP-C; ANPR-C; ANPRC; C5orf23; GUCY2B; NPR-C; NPRC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >RC219453 representing NM_000908 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTCTCTGCTGGTGCTCACTTTCTCCCCGTGCGTACTACTCGGCTGGGCGTTGCTGGCCGGCGGCA CCGGTGGCGGTGGCGTTGGCGGCGGCGGCGGTGGCGCGGGCATAGGCGGCGGACGCCAGGAGAGAGAGGC GCTGCCGCCACAGAAGATCGAGGTGCTGGTGTTACTGCCCCAGGATGACTCGTACTTGTTTTCACTCACC CGGGTGCGGCCGGCCATCGAGTATGCTCTGCGCAGCGTGGAGGGCAACGGGACTGGGAGGCGGCTTCTGC CGCCGGGCACTCGCTTCCAGGTGGCTTACGAGGATTCAGACTGTGGGAACCGTGCGCTCTTCAGCTTGGT GGACCGCGTGGCGGCGGCGCGGGGCGCCAAGCCAGACCTTATCCTGGGGCCAGTGTGCGAGTATGCAGCA GCGCCAGTGGCCCGGCTTGCATCGCACTGGGACCTGCCCATGCTGTCGGCTGGGGCGCTGGCCGCTGGCT TCCAGCACAAGGACTCTGAGTACTCGCACCTCACGCGCGTGGCGCCCGCCTACGCCAAGATGGGCGAGAT GATGCTCGCCCTGTTCCGCCACCACCACTGGAGCCGCGCTGCACTGGTCTACAGCGACGACAAGCTGGAG CGGAACTGCTACTTCACCCTCGAGGGGGTCCACGAGGTCTTCCAGGAGGAGGGTTTGCACACGTCCATCT ACAGTTTCGACGAGACCAAAGACTTGGATCTGGAAGACATCGTGCGCAATATCCAGGCCAGTGAGAGAGT GGTGATCATGTGTGCGAGCAGTGACACCATCCGGAGCATCATGCTGGTGGCGCACAGGCATGGCATGACC AGTGGAGACTACGCCTTCTTCAACATTGAGCTCTTCAACAGCTCTTCCTATGGAGATGGCTCATGGAAGA GAGGAGACAAACACGACTTTGAAGCTAAGCAAGCATACTCGTCCCTCCAGACAGTCACTCTACTGAGGAC AGTGAAACCTGAGTTTGAGAAGTTTTCCATGGAGGTGAAAAGTTCAGTTGAGAAACAAGGGCTCAATATG GAGGATTACGTTAACATGTTTGTTGAAGGATTCCACGATGCCATCCTCCTCTACGTCTTGGCTCTACATG AAGTACTCAGAGCTGGTTACAGCAAAAAGGATGGAGGGAAAATTATACAGCAGACTTGGAACAGAACATT TGAAGGTATCGCCGGGCAGGTGTCCATAGATGCCAACGGAGACCGATATGGGGATTTCTCTGTGATTGCC ATGACTGATGTGGAGGCGGGCACCCAGGAGGTTATTGGTGATTATTTTGGAAAAGAAGGTCGTTTTGAAA TGCGGCCGAATGTCAAATATCCTTGGGGCCCTTTAAAACTGAGAATAGATGAAAACCGAATTGTAGAGCA TACAAACAGCTCTCCCTGCAAATCATGTGGCCTAGAAGAATCGGCAGTGACAGGAATTGTCGTGGGGGCT TTACTAGGAGCTGGCTTGCTAATGGCCTTCTACTTTTTCAGGAAGAAATACAGAATAACCATTGAGAGGC GAACCCAGCAAGAAGAAAGTAACCTTGGAAAACATCGGGAATTACGGGAAGATTCCATCAGATCCCATTT TTCAGTAGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC219453 representing NM_000908 Red=Cloning site Green=Tags(s) MPSLLVLTFSPCVLLGWALLAGGTGGGGVGGGGGGAGIGGGRQEREALPPQKIEVLVLLPQDDSYLFSLT RVRPAIEYALRSVEGNGTGRRLLPPGTRFQVAYEDSDCGNRALFSLVDRVAAARGAKPDLILGPVCEYAA APVARLASHWDLPMLSAGALAAGFQHKDSEYSHLTRVAPAYAKMGEMMLALFRHHHWSRAALVYSDDKLE RNCYFTLEGVHEVFQEEGLHTSIYSFDETKDLDLEDIVRNIQASERVVIMCASSDTIRSIMLVAHRHGMT SGDYAFFNIELFNSSSYGDGSWKRGDKHDFEAKQAYSSLQTVTLLRTVKPEFEKFSMEVKSSVEKQGLNM EDYVNMFVEGFHDAILLYVLALHEVLRAGYSKKDGGKIIQQTWNRTFEGIAGQVSIDANGDRYGDFSVIA MTDVEAGTQEVIGDYFGKEGRFEMRPNVKYPWGPLKLRIDENRIVEHTNSSPCKSCGLEESAVTGIVVGA LLGAGLLMAFYFFRKKYRITIERRTQQEESNLGKHRELREDSIRSHFSVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_000908 |
| ORF Size | 1620 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@quimigen.pt The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000908.4 |
| RefSeq Size | 2651 bp |
| RefSeq ORF | 1623 bp |
| Locus ID | 4883 |
| UniProt ID | P17342 |
| Cytogenetics | 5p13.3 |
| Domains | ANF_receptor |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 59.6 kDa |
| Summary | This gene encodes one of three natriuretic peptide receptors. Natriutetic peptides are small peptides which regulate blood volume and pressure, pulmonary hypertension, and cardiac function as well as some metabolic and growth processes. The product of this gene encodes a natriuretic peptide receptor responsible for clearing circulating and extracellular natriuretic peptides through endocytosis of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011] |
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