NEFL High Sensitivity ELISA Kit (Human)
Referência OKCD01380
Tamanho : 96Wells
Marca : Aviva Systems Biology
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| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| COA Datasheet | ClickHereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Neurofilament, Light Polypeptide (NEFL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Neurofilament, Light Polypeptide (NEFL) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 1.56-100pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Neurofilament, Light Polypeptide (NEFL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Neurofilament, Light Polypeptide (NEFL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, cerebrospinal fluid and other biological fluids | ||||||||||||||||||||||
| Sensitivity | < 0.61 pg/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide (NEFL). No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | NEFL |
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| Gene Full Name | neurofilament light chain |
| Alias Symbols | 68 kDa neurofilament protein;CMT1F;CMT2E;CMTDIG;light molecular weight neurofilament protein;neurofilament light polypeptide;neurofilament protein, light chain;neurofilament subunit NF-L;neurofilament triplet L protein;neurofilament, light polypeptide 68kDa;NF68;NFL;NF-L;PPP1R110;protein phosphatase 1, regulatory subunit 110. |
| NCBI Gene Id | 4747 |
| Protein Name | Neurofilament light polypeptide |
| Description of Target | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. |
| Uniprot ID | P07196 |
| Protein Accession # | NP_006149.2 |
| Nucleotide Accession # | NM_006158.4 |