NLS-dCas9-EGFP-NLS
Referência PR-137213G
Tamanho : 1vial
Marca : Novatein Biosciences
Contactar o distribuidor local :
Telefone : +1 850 650 7790
NLS-dCas9-EGFP-NLS
PR-137213G
Data sheet
| Size | 50 ug |
| Storage | -70-EGFP is a fusion protein containing a nuclear localization sequence (NLS) on its N terminal and EGFP on the C terminal. The dCas9 RNP complex can localize to the nucleus immediately upon entering the cell with the addition of a nuclear localization signal (NLS). The EGFP can be taken as a reporter for tracking or sorting transfected cells, which creates the possibility of enriching cell populations for desired genome edits via fluorescence activated cell sorting (FACS). NLS-dCas9-EGFP is better than NLS-Cas9-EGFP in genome tracking by in situ hybridization, due to the inactivity of nuclease while retaining the DNA binding ability |
| Purity | Greater than 90% as determined by SDS-PAGE |
| Host/Source | E.coli |
| Concentration | 1 μM |
| Storage buffer | 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol |
| 10X Reaction Buffer | Provided |
More info
For molar concentration and other related product information, please click here:
. for Minor-Allele Enrichment in Cell-Free DNA Vol. 64, Issue 2 p307-p916
2. Purified Cas9 Fusion Proteins for Advanced Genome Manipulation Jovan Mircetic et al. Small Methods 2017 1, 1600052
3. Disruptive non-disruptive applications of CRISPR/Cas9, Volume 48 Pages 203-209
4. Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP, Issue 4 April 2016
5. High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding Evan A Boyle May, 114 (21) 5461-5466
6.CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells vol. 112 | no. 38 p11870-11875