Occludin (Marker of Early Blood Brain Barrier Damage)(OCLN/2181), 0.2mg/mL
Referência BNUB2181-500
Tamanho : 500uL
Marca : Biotium
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Antibody number | #2181 |
|---|---|
| Antibody reactivity (target) | Occludin |
| Antibody type | Primary |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone | OCLN/2181 |
| Isotype | IgG1, kappa |
| Molecular weight | 60-82 kDa |
| Synonyms | BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory subunit 115; PTORCH1; Tight junction protein occludin |
| Human gene symbol | OCLN |
| Entrez gene ID | 100506658 |
| SwissProt | Q16625 |
| Unigene | 592605 |
| Immunogen | Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary) |
| Verified antibody applications | IHC (FFPE) (verified), WB (verified) |
| Antibody target cellular localization | Cell junctions, Plasma membrane |
| Species reactivity | Human |
| Positive control | HepG2 cells. Kidney. |
| Shipping condition | Room temperature |
| Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C |
| Regulatory status | For research use only (RUO) |
| Antibody/conjugate formulation | Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
| Cell/tissue expression | Microvascular endothelial cells |
Product Description
Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB). Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the
CF® Dye Brochure
for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.info@quimigen.pt
to inquire about stock status and lead times before placing your order.Catalog number key for antibody number 2181, Anti-Occludin (OCLN/2181)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
|---|---|---|---|---|---|
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified, BSA-free | N/A | N/A | N/A |