Phosphate Colorimetric Assay Kit(Malachite Green Method)

Referência E-BC-K832-M-96T

Tamanho : 96T

Marca : Elabscience

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Detection Principle Phosphorus is an important mineral, one of the essential nutrients for all animals, and is essential for maintaining phosphate homeostasis.
Malachite green method is a sensitive method for phosphate analysis. The principle is that the compounds formed by phosphoric acid and molybdate under acidic conditions will form color substances with malachite green. The depth of color is proportional to the content of phosphate. The phosphorus content can be calculated indirectly be measuring the OD value at 636 nm.
Sample Type Serum,Plasma,Tissue,Cell
Detection Method Colorimetric method
Detection Instrument Microplate reader (630-650 nm)
Research Area Inorganic Ions ,   Oxidative Stress
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.001 mmol/L
Detection Range 0.001-0.20 mmol/L
Precision inter-assay CV: 0.08 | intra-assay CV: 0.04
Assay Time 35 min
Cat.No. Product Name Sizes
E-BC-K831-M ATPase Activity Assay Kit 96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Rat liver tissue homogenate 15-25
10% Rat heart tissue homogenate 15-25
10% Rat spleen tissue homogenate 15-25
10% Rat brain tissue homogenate 15-25
10% Rat kidney tissue homogenate 15-25
10% Mouse heart tissue homogenate 15-25
10% Mouse lung tissue homogenate 15-25
10% Mouse ovarian tissue homogenate 50-70
Rat serum 8-12
Rat plasma 8-12
Human plasma 8-12

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

Other Clones

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Other Formats

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® Violet 450

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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Referência
Descrição
Cond.
Price Bef. VAT
230-30116-100
 100ug 
8118-SC
 2500tests