Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue, which makes it soluble in water.
The substance is a nonspecific attachment factor for cells. It promotes cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and positively charged ions on the culture surface.
For coating of a 25 cm² culture dish it is recommended to use 1.0 ml of a 0.1 mg/ml solution. Remove the solution after 5 minutes through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.
Can also be used for coating of glass coverslips.