Pyruvate Kinase BioAssay™ Kit (PK)

Pyruvate Kinase (PK) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase deficiency, a genetic disease, is caused by a lack of pyruvate kinase and slows down the process of glycolysis. Pyruvate kinase is also involved in gluconeogenesis, a biochemical pathway in which the liver generates glucose from pyruvate and other substrates. The Pyruvate Kinase Assay Kit provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity. In this assay PEP and ADP are catalyzed by pyruvate kinase to generate pyruvate and ATP. The color intensity of the reaction product at 570nm or fluorescence intensity at lex/em=530/590nm is directly proportional to|the pyruvate generated by the Pyruvate Kinase in the sample.||Intended Use:|For quantitative determination of pyruvate kinase activity and evaluation of drug effects on PK activity.||Applications:|Direct Assays: PK activity levels in plasma, serum, and tissue samples.|Drug Discovery/Pharmacology: effects of drugs on PK activity.||Range:|0.1-50U/L for colorimetric assays and 0.01-2U/L for fluorometric assays||Detection Limit: |0.01U/L||Kit Components:|Developer, 1x12ml|Dye Reagent, 1x120ul|Cosubstrate, 1x120ul|Pyruvate Standard, 1x400ul 25mM||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.