General Info
| Applications: | ELISA |
| Reactivity: | Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.116ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description: | This ACO1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
Information
| Gene Symbol: | Aco1 |
| Gene ID: | 50655 |
| Uniprot ID: | ACOHC_RAT |
| Sample Type: | tissue homogenates or other biological fluids. |
Description
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA. Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate. |
| Protein Name | Cytoplasmic Aconitate HydrataseAconitaseCitrate Hydro-LyaseIron Regulatory Protein 1Irp1Iron-Responsive Element-Binding Protein 1Ire-Bp 1 |
| Database Links | Reactome: R-RNO-917937 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative ELISA Names | Cytoplasmic Aconitate Hydratase ELISA kitAconitase ELISA kitCitrate Hydro-Lyase ELISA kitIron Regulatory Protein 1 ELISA kitIrp1 ELISA kitIron-Responsive Element-Binding Protein 1 ELISA kitIre-Bp 1 ELISA kitAco1 ELISA kitIreb1 ELISA kitIrebp ELISA kit |
| output |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance