600222 | Rat IFN-gamma Quimigen Portugal

Recombinant Active Protein

Cat. No.

600222

Unit

0.1 mg

Description

Interferon gamma (IFN-gamma) is the major interferon produced by stimulated lymphocytes. IFN-gamma is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. IFN-gamma is mainly produced by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. The synthesis of IFN-gamma is induced by Interleukin-2, FGF-basic, and EGF. Recombinant Rat IFN-gamma produced in E. coli is a single non-glycosylated polypeptide chain containing 135 amino acids with a MW of 15,609 Da.

Spec Container

Datasheet

Format

Each vial contains 100 ug protein lyophilized in 0.5X PBS pH7.2. Reconstitute with 1.0 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Alternate Names

interferon gamma; IFN-gamma; immune interferon; IFNG

Accession No.

P01581

15609 Da

Sequence

MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

Recombinant Rat Interferon gamma is fully biologically active when compared to standard. The specific activity as determined in a viral challenge assay using EMC virus on L929, corresponding to an ED50 of 0.8 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

Images

For research use only, not for diagnostic or therapeutic procedures.

Rat IFN-gamma

Referência 600222

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Marca : Abbiotec

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Spec Container