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> Rat IgG1 Antibody : APC

Rat IgG1 Antibody : APC

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Referência OASB00093

Tamanho : 0.1mg

Marca : Aviva Systems Biology

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Rat IgG1 Antibody : APC (OASB00093)

IgG1-UNLB, IgG2a-UNLB, IgG2b-UNLB, IgG2c-UNLB, and IgM-UNLB
Datasheets/ManualsPrintable datasheet for Rat IgG1 Antibody : APC (OASB00093)
Product Info
ClonalityMonoclonal
CloneKLH/G1-2-2
IsotypeIgG1
HostRat
ConjugationAPC
ApplicationFC, FLISA
Reconstitution and StorageStore at 2-8C
Concentration0.1 mg/mL
CharacterizationTo insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance with characteristics of a standard reference reagent.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionFlow Cytometry:
ELISA:
Fluorescein conjugate
Biotin conjugate
R-phycoerythrin conjugate
Allophycocyanin conjugate
Spectral Red conjugate
R-phycoerythrin-Cyanine 5.5 conjugate
R-phycoerythrin-Cyanine 7 conjugate
Allophycocyanin-Cyanine 5.5 conjugate
Allophycocyanin-Cyanine 7 conjugate
Purified antibody
AP conjugate
HRP conjugate
<=1 ug/106 cells
<=1 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
1ug/mL
1:2,000-1:4,000
1:4,000-1:8,000
Application InfoFlow cytometry, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA)
Other Applications DataSince applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs.
Storage- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C. 20 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD), R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5), R- phycoerythrin- Cyanine 7(R- PE- CY 7), Allophycocyanin- Cyanine 5.5 (APC- CY 5.5), and Allophycocyanin- Cyanine 7 (APCCY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
- With the exception of the low endotoxin, azide- free product, the vials should be stored at 2- 8 C
Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquotand freeze the low endotoxin, azide- free product at - 20 C immediately upon receipt. Each reagent isstable for the period shown on the bottle label if stored as directed.
Gene Full NameIg gamma-1 chain C region