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Rat IgG1-FITC
Marca : Southern Biotech
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Rat IgG1-FITC (KLH/G1-2-2)
Cat. No.:
0116-02
Rat IgG1-FITC for use as a control in flow cytometry and immunocytochemistry assays.
Clone | KLH/G1-2-2 |
---|---|
Isotype | Rat IgG1κ |
Specificity | KLH |
Conjugate | FITC (Fluorescein) |
Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
Clonality | Monoclonal |
Concentration | 0.5 mg/mL |
Volume | 1.0 mL |
Recommended Storage | 2-8°C; Avoid exposure to light |
Applications | Flow Cytometry – Quality tested 1-3 ELISA – Quality tested FLISA – Quality tested Immunocytochemistry – Reported in literature 3,4 Blocking – Reported in literature 5 In vitro Control – Reported in literature 6-8 In vivo Control – Reported in literature 8-14 |
RRID Number | AB_2793980 |
Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup
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UNLB AP HRP BIOT PE APC SPRD LE/AF PE/CY7 APC/CY5.5 APC/CY7 PACBLU AF488 AF647
- 1. Chu VT, Fröhlich A, Steinhauser G, Scheel T, Roch T, Fillatreau S, et al. Eosinophils are required for the maintenance of plasma cells in the bone marrow. Nat Immunol. 2011;12:151-60. (FC)
- 2. Berge T, Grønningsæter IH, Lorvik KB, Abrahamsen G, Granum S, Sundvold-Gjerstad V, et al. SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice. PLoS One. 2012;7(10):e48239. (FC)
- 3. Gaspar-Elsas MI, Queto T, Vasconcelos Z, Jones CP, Lannes-Vieira J, Xavier-Elsas P. Evidence for a regulatory role of α4-integrins in the maturation of eosinophils generated from the bone marrow in the presence of dexamethasone. Clin Exp Allergy. 2009;39:1187-98. (FC, ICC)
- 4. Chu VT, Berek C . Immunization induces activation of bone marrow eosinophils required for plasma cell survival. Eur J Immunol. 2012;42:130-7. (ICC)
- 5. Talkington J, Nickell SP. Role of Fc gamma receptors in triggering host cell activation and cytokine release by Borrelia burgdorferi. Infect Immun. 2001;69:413-9. (Block)
- 6. Lopez JI, Camenisch TD, Stevens MV, Sands BJ, McDonald J, Schroeder JA. CD44 attenuates metastatic invasion during breast cancer progression. Cancer Res. 2005;65:6755-63. (In vitro control)
- 7. Lee S, Choi H, Eun S, Fukuyama S, Croft M. Nitric oxide modulates TGF-β-directive signals to suppress Foxp3+ regulatory T cell differentiation and potentiate Th1 development. J Immunol. 2011;186:6972-80. (In vitro control)
- 8. Brouxhon SM, Kyrkanides S, Teng X, Raja V, O'Banion MK, Clarke R, et al. Monoclonal antibody against the ectodomain of E-cadherin (DECMA-1) suppresses breast carcinogenesis: involvement of the HER/PI3K/Akt/mTOR and IAP pathways. Clin Cancer Res. 2013;19:3234-46. (In vitro control, in vivo control)
- 9. Haeryfar SM, DiPaolo RJ, Tscharke DC, Bennink JR, Yewdell JW. Regulatory T cells suppress CD8+ T cell responses induced by direct priming and cross-priming and moderate immunodominance disparities. J Immunol. 2005;174:3344-51. (In vivo control)
- 10. Liang Y, Christopher K, Finn PW, Colson YL, Perkins DL. Graft produced interleukin-6 functions as a danger signal and promotes rejection after transplantation. Transplantation. 2007;84:771-7. (In vivo control)
- 11. Dakhama A, Lee Y, Ohnishi H, Jing X, Balhorn A, Takeda K, et al. Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice. J Allergy Clin Immunol. 2009;123:138-45. (In vivo control)
- 12. Zhang Z, Li Z, Yan S, Wang X, Abecassis M. TNF-α signaling is not required for in vivo transcriptional reactivation of latent murine cytomegalovirus. Transplantation. 2009;88:640-5. (In vivo control)
- 13. Saha S, Jing X, Park SY, Wang S, Li X, Gupta D. Peptidoglycan recognition proteins protect mice from experimental colitis by promoting normal gut flora and preventing induction of interferon-γ. Cell Host Microbe. 2010;8:147-62. (In vivo control)
- 14. Brouxhon SM, Kyrkanides S, Raja V, Silberfeld A, Teng X, Trochesset D, et al. Ectodomain-specific E-cadherin antibody suppresses skin SCC growth and reduces tumor grade: a multitargeted therapy modulating RTKs and the PTEN-p53-MDM2 axis. Mol Cancer Ther. 2014;13:1791-802. (In vivo control)