Rat IgM Antibody : APC
Referência OASB00138
Tamanho : 0.1mg
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
Rat IgM Antibody : APC (OASB00138)
| Datasheets/Manuals | Printable datasheet for Rat IgM Antibody : APC (OASB00138) |
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| Clonality | Monoclonal |
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| Clone | NIP/M-2 |
| Isotype | IgM |
| Host | Rat |
| Conjugation | APC |
| Application | FC, FLISA |
| Reconstitution and Storage | Store at 2-8C |
| Concentration | 0.1 mg/mL |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested to conform to characteristics of a standardreference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flowcytometer and/or ELISA. Representative data are included in this Technical Bulletin. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugates Biotin conjugate R-phycoerythrin conjugate Allophycocyanin conjugate Spectral Red conjugate R-phycoerythrin-Cyanine 7 conjugate <=1 ug/106 cells <=1 ug/106 cells <=0.1 ug/106 cells <=0.1 ug/106 cells <=0.1 ug/106 cells <=0.1 ug/106 cells |
| Application Info | Flow cytometry, Enzyme-Linked-Immunosorbent-Assay (ELISA) |
| Other Applications Data | Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs. |
| Other Applications Image 1 Data | Amount Used: Ug/106 cells BALB/c spleen cells were incubated with either PBS or rat IgM-FITC, following which small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) and fluorescein- 5- maleimide (FLMA) conjugates are supplied as 0.5 mg in 1.0 mLPBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 7 (R- PE- CY 7) conjugates are supplied as0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - With the exception of the low endotoxin, azide- free product, the vials should be stored at 2- 8 C Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquotand freeze the low endotoxin, azide- free product at - 20 C immediately upon receipt. Each reagent isstable for the period shown on the bottle label if stored as directed. |
| Gene Full Name | Ig mu chain C region |
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