Rat TNF alpha ELISA Kit
Referência EA100366
Tamanho : 1x96wells
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| Product Data | |
| ACCN | NM_012675 |
|---|---|
| Assay Length | 3.5 hours incubations; 1 hour washing and analyzing samples |
| Assay Platform | 96-well plate |
| Assay Type | Sandwich ELISA kit of Quantitative Detection for Rat TNF alpha |
| Assay Type | Sandwich |
| Background | Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa. |
| Components |
|
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Curve Range | 15.6 pg/ml - 1000 pg/ml |
| Gene Symbol | Tnf |
| Interference | No significant interference observed with available related molecules. |
| Locus ID | 24835 |
| Reactivity | Rat |
| Reactivity | Rat |
| Reactivity | Rat |
| Sample Type | Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sample Volume | 100 ul |
| Sensitivity | <1pg/ml |
| Signal | Colorimetric |
| Species | Rat |
| Specificity | This kit is used for quantitative detection of rat TNF alpha |
| Target Symbol | Tnf |
| Description | Rat TNF alpha ELISA Kit |
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