TP303507 | Seladin 1 (DHCR24) (NM_014762) Human Recombinant Protein

Seladin 1 (DHCR24) (NM_014762) Human Recombinant Protein

SKU

TP303507

Recombinant protein of human 24-dehydrocholesterol reductase (DHCR24), 20 µg

MVPro

Full-length human proteins expressed in HEK293T cells

Specifications
Product Data
Species	Human
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence >RC203507 representing NM_014762 Red=Cloning site Green=Tags(s) MEPAVSLAVCALLFLLWVRLKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRLHEQR VRDIQKQVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKTHKNIMINLMDILEVDTKKQIVRVEPLVTMG QVTALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRCTPSENSDL FYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGLEAICAKFTHESQRQENHFVEGLLYSLDEAVIM TGVMTDEAEPSKLNSIGNYYKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPI FRYLFGWMVPPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFILPSQ PGLVHPKGNEAELYIDIGAYGEPRVKHFEARSCMRQLEKFVRSVHGFQMLYADCYMNREEFWEMFDGSLY HKLREKLGCQDAFPEVYDKICKAARH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Predicted MW	57.6 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation	Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping	Dry Ice
Reference Data
RefSeq	NP_055577
Locus ID	1718
UniProt ID	Q15392
Cytogenetics	1p32.3
RefSeq Size	4286
RefSeq ORF	1548
Synonyms	DCE; Nbla03646; seladin-1; SELADIN1
Summary	This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transmembrane
Protein Pathways	Metabolic pathways, Steroid biosynthesis

FAQs
Protein FAQs
SDS
SDS for TP303507

Recombinant Protein Resources
Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

SKU	Description	Size
PH303507	DHCR24 MS Standard C13 and N15-labeled recombinant protein (NP_055577)	10 ug
LC402373	DHCR24 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug
LY402373	Transient overexpression lysate of 24-dehydrocholesterol reductase (DHCR24)	100 ug

Seladin 1 (DHCR24) (NM_014762) Human Recombinant Protein

Referência TP303507

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Seladin 1 (DHCR24) (NM_014762) Human Recombinant Protein

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