Sirt3, Fluorogenic Assay Kit, BioAssay™ (Sirtuin 3, SIR2L3)

The Fluorogenic SIRT3 Assay Kit is a complete assay system designed to measure Sirtuin 3 (SIRT3) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary. The kit includes purified SIRT3 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT3 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT3 activity level. First, the HDAC fluorometric substrate (HDAC substrate 1), containing an acetylated lysine side chain, is incubated with purified SIRT3 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.||Intended Use:|Designed to study enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.||Kit Components:|*137371A: SIRT3 recombinant human enzyme, 1x70ug|*137371B: Fluorogenic HDAC substrate 1 (5mM), 1x50ul |*137371C: Nicotinamide Adenine Dinucleotide (NAD+) 50mM, 1x50ul|*137371D: Nicotinamide (10mM), 1x500ul|*137371E: SIRT Developer 2X (contains 2mM Nicotinamide), 1x6ml|**137371F: SIRT Assay Buffer, 1x10ml|***137371G: Black, low binding NUNC black microtiter plate, 1x32 wells||Storage and Stability:|Store *137371A, *137371B, *137371C, *137371D and *137371E at -70°C. Store **137371F at -20°C. ***137371G at RT. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.