Smarcb1 Mouse Gene Knockout Kit (CRISPR)
Referência KN316287LP
Tamanho : 1kit
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Smarcb1 Mouse Gene Knockout Kit (CRISPR)
| Product Data | |
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
|---|---|
| Vector | Luciferase-Puro |
| Target Symbol | Smarcb1 |
| Locus ID | 20587 |
| Components | KN316287G1, Smarcb1 gRNA vector 1 in pCas-Guide CRISPR vector KN316287G2, Smarcb1 gRNA vector 2 in pCas-Guide CRISPR vector KN316287LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| OTI Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001161853, NM_011418 |
| UniProt ID | Q9Z0H3 |
| Synonyms | AU020204; Baf47; Ini1; Snf5; SNF5/INI1 |
| Summary | Core component of the BAF (SWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.[UniProtKB/Swiss-Prot Function] |
| Shipping | Ambient |
More linear donor DNA can be ordered separately
| Cat# | KN316287LPD |
| Description | 10 ug linear donor DNA for KN316287LP kit |
| /€515 |
If you want to order 50ug, please order quantity of 5 for KN316287LPD
Other selection marker is available for donor DNA.
Please contact tech@quimigen.pt for a custom quote.
| Product Manuals |
| FAQs |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| GA203980 | Smarcb1 CRISPRa kit - CRISPR gene activation of mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 | 1 kit | | |
| KN316287 | Smarcb1 - mouse gene knockout kit via CRISPR, HDR mediated | 1 kit | | |
| KN316287BN | Smarcb1 - mouse gene knockout kit via CRISPR, HDR mediated | 1 kit | | |
| KN316287RB | Smarcb1 - mouse gene knockout kit via CRISPR, HDR mediated | 1 kit | | |
| KN516287 | Smarcb1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 kit | | |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.