Sucrose Assay Kit is an assay kit designed for the detection and quantification of Sucrose concentrations in plant tissue using a spectrophotometer. Sucrose in plant tissue is hydrolyzed to glucose and fructose under acidic and heat conditions. 5-hydroxymethylfurfural can be synthesized from fructose under acid conditions, the concentration of which can be determined by measuring the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption corresponding to glucose is much smaller than fructose.
Research Articles on Sucrose
| Target | Sucrose |
| Reactivity | General (All species) |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Validity | The validity for this kit is 12 months. |
| Test Range | 0.32 µmol/ml - 70 µmol/ml |
| Sensitivity | 0.32 µmol/ml |
| Detection Method | Colorimetric |
| Assay Data | Quantitative |
| Sample Type | Plant tissue. |
| CAS Number | 57-50-1 |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |