Assay Principle:

The DetectX minutes, is designed for quick and efficient analysis. For comprehensive guidance, users should read the complete kit insert before conducting the assay. The kit includes a Superoxide Dismutase Standard to establish an accurate standard curve.

Protocol Summary:

• Place standards or diluted samples into the provided transparent microtiter plate.
• Add Substrate Preparation mixture and Xanthine Oxidase Preparation mixture to each well, ensuring thorough mixing.
• Incubate the mixture at room temperature for 20 minutes. This allows for the color-generating reaction between Xanthine Oxidase, oxygen, and the colorless substrate.
• After incubation, use a plate reader to detect the generated signal at 450nm and calculate SOD activity using the standard curve.

Background:

Superoxide dismutases (SODs) are key enzymes in the body’s antioxidant defense system, scavenging harmful oxygen species such as superoxide (O2-O2). These enzymes, including copper-zinc superoxide dismutase (Cu/ZnSOD), manganese superoxide dismutase (MnSOD), and extracellular superoxide dismutase (EC-SOD), are critical in controlling cellular ROS levels. Reduced SOD activity can lead to elevated superoxide levels, impacting NO availability and increasing peroxynitrite concentrations.

Cu/Zn SOD, a 32-kDa homodimer containing copper and zinc, is the primary intracellular SOD. MnSOD, found in mitochondria, is a manganese-containing 93-kDa homotetramer synthesized in the cytoplasm and transported to the mitochondrial matrix. Many organs highly express EC-SOD, the main extracellular SOD enzyme. Altered SOD activity levels are associated with several conditions, including Down Syndrome, diabetes, Alzheimer id="related-products-heading">Related News