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> Tachysterol 3 [17592-07-3]

Tachysterol 3 [17592-07-3]

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Referência HY-130705A-5mg

Tamanho : 5mg

Marca : MedChemExpress

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Tachysterol 3 is a photoproduct of Previtamin D3 (HY-130705).

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Tachysterol 3 Chemical Structure

Tachysterol 3 Chemical Structure

CAS No. : 17592-07-3

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Other Forms of Tachysterol 3:

  • Previtamin D3 Obtenir un devis
Description

Tachysterol 3 is a photoproduct of Previtamin D3 (HY-130705)[1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Tachysterol 3 can be hydroxylated by CYP11A1 and CYP27A1[2].
Tachysterol 3 is detected in human serum at a concentration of 7.3 ± 2.5 ng/mL[2].
Tachysterol 3 hydroxyderivatives show high-affinity binding to the ligan-binding domain (LBD) of the liver X receptor (LXR) α and β, and the peroxisome proliferator-activated receptor γ (PPARγ) in LanthaScreen TR-FRET coactivator assays[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tachysterol 3 Related Antibodies
Masse moléculaire

384.64

Formule

C27H44O
CAS No.

17592-07-3
Appearance

Oil

Color

Colorless to light yellow

SMILES

O[C@@H]1CC(/C=C/C2=CCC[C@]3(C)[C@@H]([C@H](C)CCCC(C)C)CC[C@@]2320)=C(C)CC1
Structure Classification
  • Others
Initial Source
  • Endogenous metabolite
Livraison

Shipping with dry ice.
Stockage

-80°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvant et solubilité
In Vitro: 

DMSO : 2 mg/mL (5.20 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5998 mL 12.9992 mL 25.9983 mL
5 mM 0.5200 mL 2.5998 mL 5.1997 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3.25 mg/mL (8.45 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3.25 mg/mL (8.45 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation

Purity: 99.68%

SDS (480 KB)

COA (246 KB) HNMR (466 KB) RP-HPLC (294 KB)

Instruction de manipulation (2659 KB)
Références

  • [1]. Cecilia Cisneros, et al. The Role of Tachysterol in Vitamin D Photosynthesis - A Non-Adiabatic Molecular Dynamics Study. Phys Chem Chem Phys. 2017 Feb 22;19(8):5763-5777.  [Content Brief]

    [2]. Slominski AT, et al. Metabolic activation of tachysterol3 to biologically active hydroxyderivatives that act on VDR, AhR, LXRs, and PPARγ receptors. FASEB J. 2022 Aug;36(8):e22451.  [Content Brief]

  • [1]. Cecilia Cisneros, et al. The Role of Tachysterol in Vitamin D Photosynthesis - A Non-Adiabatic Molecular Dynamics Study. Phys Chem Chem Phys. 2017 Feb 22;19(8):5763-5777.

    [2]. Slominski AT, et al. Metabolic activation of tachysterol3 to biologically active hydroxyderivatives that act on VDR, AhR, LXRs, and PPARγ receptors. FASEB J. 2022 Aug;36(8):e22451.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5998 mL 12.9992 mL 25.9983 mL 64.9958 mL
5 mM 0.5200 mL 2.5998 mL 5.1997 mL 12.9992 mL
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Tachysterol 3 Related Classifications

  • Metabolic Disease
  • Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease
  • VD/VDR Endogenous Metabolite
Help & FAQs

Keywords:

Tachysterol 317592-07-3Tachysterol3Tachysterol-3VD/VDREndogenous MetaboliteVitamin DVitamin D receptorvitamin Dprevitamin D3CYP11A1CYP27A1human serumInhibitorinhibitorinhibit

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