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TDP43 staining of formalin fixed adult rat brain, specifically the hippocampus; whose neurons nuclei are stained strongly, nuclei of some non-neuroal cells are weakly stained. Neuronal nuclei therefore look crimson, since they are both red due to the content of TDP43 and blue due to their content of DNA, stained blue with DAPI. Chicken antibody to GFAP (G) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (B).
TDP43 staining of formalin fixed adult rat brain, specifically the hippocampus; whose neurons nuclei are stained strongly, nuclei of some non-neuroal cells are weakly stained. Neuronal nuclei therefore look crimson, since they are both red due to the content of TDP43 and blue due to their content of DNA, stained blue with DAPI. Chicken antibody to GFAP (G) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (B).
Blots of TDP43 on crude extract of mouse brain nuclear fraction (left lane) and cytoplasmic fraction (right lane). There is a strong clear band in the nuclear preparation running at 43kDa, and a much more minor band in the cytoplasmic fraction. Image courtesy of Irina Madorsky. Protocol on data-sheet.
Blots of TDP43 on crude extract of mouse brain nuclear fraction (left lane) and cytoplasmic fraction (right lane). There is a strong clear band in the nuclear preparation running at 43kDa, and a much more minor band in the cytoplasmic fraction. Image courtesy of Irina Madorsky. Protocol on data-sheet.