Tardbp (NM_145556) Mouse Tagged ORF Clone
Referência MG206534
Tamanho : 10µg
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| Product Data | |
| Target Symbol | Tardbp |
|---|---|
| Synonyms | 1190002A23Rik; C85084; TDP-43; Tdp43 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >MG206534 representing NM_145556 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAATATATTCGGGTAACAGAAGATGAGAACGATGAACCCATTGAAATACCATCAGAAGACGATG GGACGGTGTTGCTGTCCACAGTTACAGCCCAGTTTCCAGGGGCATGCGGCCTGCGCTACCGGAATCCCGT GTCTCAGTGTATGAGAGGAGTCCGACTGGTGGAAGGAATTCTGCATGCCCCAGATGCTGGCTGGGGCAAT CTGGTATATGTTGTCAACTATCCCAAAGATAACAAAAGGAAAATGGATGAGACAGATGCTTCCTCTGCAG TGAAAGTGAAAAGAGCAGTCCAGAAAACATCTGACCTCATAGTGTTGGGTCTCCCCTGGAAAACAACTGA GCAGGATCTGAAAGACTATTTCAGTACTTTTGGAGAGGTTCTTATGGTTCAGGTCAAGAAAGATCTTAAA ACTGGTCACTCGAAAGGGTTTGGCTTTGTTCGATTTACAGAATATGAAACCCAAGTGAAAGTAATGTCAC AACGACATATGATAGATGGGCGATGGTGTGACTGTAAACTTCCCAACTCTAAGCAAAGCCCAGACGAGCC TTTGAGAAGCAGAAAGGTGTTTGTTGGACGTTGTACAGAGGACATGACTGCTGAAGAGCTTCAGCAGTTT TTCTGTCAGTATGGAGAAGTGGTAGATGTCTTCATTCCCAAACCATTCAGAGCTTTTGCCTTCGTCACCT TTGCAGATGATAAGGTTGCCCAGTCTCTTTGTGGAGAGGATTTGATCATTAAAGGAATCAGCGTGCATAT ATCCAATGCTGAACCTAAGCATAATAGCAATAGACAGTTAGAAAGAAGTGGAAGATTTGGTGGTAATCCA GGTGGCTTTGGGAATCAGGGTGGGTTTGGTAACAGTAGAGGGGGTGGAGCTGGCTTGGGAAATAACCAGG GTGGTAATATGGGTGGAGGGATGAACTTTGGTGCTTTTAGCATTAACCCAGCGATGATGGCTGCGGCTCA GGCAGCGTTGCAGAGCAGTTGGGGTATGATGGGCATGTTAGCCAGCCAGCAGAACCAGTCGGGCCCATCT GGGAATAACCAAAGCCAGGGCAGCATGCAGAGGGAACCAAATCAGGCTTTTGGTTCTGGAAATAATTCCT ACAGTGGTTCTAATTCTGGTGCCCCCCTTGGTTGGGGGTCAGCATCAAATGCAGGATCGGGCAGTGGTTT TAATGGGGGCTTTGGCTCGAGCATGGATTCTAAGTCTTCTGGCTGGGGAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence >MG206534 representing NM_145556 Red=Cloning site Green=Tags(s) MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGN LVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKDYFSTFGEVLMVQVKKDLK TGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSPDEPLRSRKVFVGRCTEDMTAEELQQF FCQYGEVVDVFIPKPFRAFAFVTFADDKVAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNP GGFGNQGGFGNSRGGGAGLGNNQGGNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPS GNNQSQGSMQREPNQAFGSGNNSYSGSNSGAPLGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_145556 |
| ORF Size | 1242 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145556.4 |
| RefSeq Size | 7457 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 230908 |
| UniProt ID | Q921F2 |
| Cytogenetics | 4 E2 |
| Summary | RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3' UTR of mRNAs. In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases. Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts. Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3' UTR leading to poly(A) tail deadenylation and thus shortening. In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival. Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins. Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed:27123980).[UniProtKB/Swiss-Prot Function] |
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