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> TCEP HCl [51805-45-9]

TCEP HCl [51805-45-9]

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Referência 10-2961-5g

Tamanho : 5g

Marca : Focus Biomolecules

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TCEP HCl | Reducing agent

CAS:
51805-45-9
Catalog Number:
10-2961
Activity:
Reducing agent
Chemical Name:
Tris(-Carboxyethyl)phosphine HCl
Molecular Weight:
286.65
Molecular Formula:
C9H15O6P • HCl
Solubility:
Soluble in Water (up to 50 mg/ml)
Physical Properties:
White solid
Purity:
99% by TLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in distilled water may be stored at -20°C for up to 1 month.
Shipping Code:
RT

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1 gram$35.00
5 gram$150.00
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TCEP HCl (51805-45-9) is a reagent for selective reduction of disulfides in aqueous solutions1-3 with distinct advantages over DTT which are application specific3. For example, spin labels are more stable with TCEP than DTT, Ni2+ (leached from affinity columns) causes rapid oxidation of DTT with no effect on TCEP and for long term storage of proteins TCEP is more stable than DTT with no metal chelator (EGTA) present but DTT is more stable in the presence of metal chelators.3

References/Citations:

1) Fischer et al. (1993), In situ reduction suitable for matrix-assisted laser desorption/ionization and liquid secondary ionization using tris(2-carboxyethyl)phosphine; Rapid Commun. Mass Spectrom., 7 225
2) Kirley et al. (1989), Reduction and fluorescent labeling of cyst(e)ine-containing proteins for subsequent structural analysis; Anal. Biochem., 180 231
3) Getz et al. (1999), A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry; Anal. Biochem., 273 73

CAS:
51805-45-9
Catalog Number:
10-2961
Activity:
Reducing agent
Chemical Name:
Tris(-Carboxyethyl)phosphine HCl
Molecular Weight:
286.65
Molecular Formula:
C9H15O6P • HCl
Solubility:
Soluble in Water (up to 50 mg/ml)
Physical Properties:
White solid
Purity:
99% by TLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in distilled water may be stored at -20°C for up to 1 month.
Shipping Code:
RT

Safety Data Sheet:

Download Safety Data Sheet

Product Data Sheet:

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Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications. Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a restocking/refund fee

TCEP HCl (51805-45-9) is a reagent for selective reduction of disulfides in aqueous solutions1-3 with distinct advantages over DTT which are application specific3. For example, spin labels are more stable with TCEP than DTT, Ni2+ (leached from affinity columns) causes rapid oxidation of DTT with no effect on TCEP and for long term storage of proteins TCEP is more stable than DTT with no metal chelator (EGTA) present but DTT is more stable in the presence of metal chelators.3

References/Citations:

1) Fischer et al. (1993), In situ reduction suitable for matrix-assisted laser desorption/ionization and liquid secondary ionization using tris(2-carboxyethyl)phosphine; Rapid Commun. Mass Spectrom., 7 225
2) Kirley et al. (1989), Reduction and fluorescent labeling of cyst(e)ine-containing proteins for subsequent structural analysis; Anal. Biochem., 180 231
3) Getz et al. (1999), A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry; Anal. Biochem., 273 73

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