TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm.
TMB X-tnd is developed to support assays, which require low detection limits while supporting OD readings of highly positive samples – with no precipitation of the TMB substrate, at up to 5.8 mAbs within 60 minutes.
TMB X-tnd® characteristics are:
Best dynamic range
No precipitation
Aqueous based – no organic solvents (REACH Annex XVII compliant)
Low lot-to-lot variation
Suitable for use in electrochemical assays
The product is available in bulk quantities and as customized solutions.