Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Referência E-BC-K020-M-96T

Tamanho : 96T

Marca : Elabscience

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Telefone : +1 850 650 7790

Detection Principle The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.
Detection Principle Image
Synonyms T-SOD
Sample Type Serum,Plasma,Hydrothorax,ascites,Urine,Cell,Tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (440-460 nm, optimum wavelength: 450 nm)
Research Area Oxidative Stress ,  Plant Physiology Series ,  Ferroptosis
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period 12 months
Sensitivity 0.2 U/mL
Detection Range 0.2-14.4 U/mL
Precision inter-assay CV: 3.7% | intra-assay CV: 2.9%
Assay Time 30 min
Cat.No. Product Name Sizes
E-BC-K025-M Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method) 500Assays , 96T , 48T
E-BC-K031-M Catalase (CAT) Activity Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 3-5
Rat serum 20-30
Urine 1
Human hydrothorax 2
Cell culture supernatant 2-3
10% Rat liver tissue homogenization 340-370
10% Rat heart tissue homogenization 80-100
10% Rat kidney tissue homogenization 100-120
10% Rat brain tissue homogenization 50-100
HepG2 cells homogenization (3 mgprot/mL) 30-40
10% Plant tissue homogenization 5-10

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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