UltraBlock-BSA™ Blocking Buffer 'Ready to Use'

Referência B395-100

Tamanho : 100ml

Marca : Leinco Technologies

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UltraBlockBSA™ Blocking Buffer ‘Ready to Use’

Product No.: B395

[product_table name="All Top" skus="B395"]

Product Type
Substrate and Assay Reagents
Applications
ELISA
,
WB

Product Details

Storage and Handling
Store at 28° C. Do Not Freeze
Country of Origin
USA
Shipping
Next Day 28°C
Expiration Date
Stable for a minimum of 2 years after receipt when properly stored at 28°C

Description

Background
Protein containing solutions are commonly used in ELISA and other immunoassays to block excess binding sites and reduce nonspecific binding of secondary reagents. Bovine Serum Albumin (BSA) has long been the choice for many scientists attempting to reduce nonspecific binding in immunoassays. UltraBlockBSATM utilizes special chemistry to enhance the solubility and stabilize the BSA. UltraBlockBSATM readily blocks and excess reagent easily washes away providing ideal blocking without lingering residual protein that can interfere with optimal signal. An additional benefit of UltraBlockBSATM is that it provides a long term stabilizing environment for the antigen, or antibody coated on the ELISA plate. long been the choice for many scientists attempting to reduce nonspecific binding in immunoassays. UltraBlockBSATM utilizes special chemistry to enhance the solubility and stabilize the BSA. UltraBlockBSATM readily blocks and excess reagent easily washes away providing ideal blocking without lingering residual protein that can interfere with optimal signal. An additional benefit of UltraBlockBSATM is that it provides a long term stabilizing environment for the antigen, or antibody coated on the ELISA plate.
Benefits
• Ease of Use – A one component “Ready Use” formulation increases assay efficiency.
• Extended Shelf Life – UltraBlockBSATM is stable for a minimum of 2 years when stored at 2° 8°C.
Directions for Use
UltraBlockBSATM is a ready to use solution that needs no preparation or dilution. Once you have coated your micro ELISA plate(s) with capture antibody or antigen, aspirate or dump out the coating solution and wash the plate 24 times. Pipette a volume greater than the coating solution volume, but not to exceed 350 µl of UltraBlockBSATM per well and let incubate for 2 – 3 hours in an incubator at 37°C.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Related Protocols

Elisa Sandwich Protocol
General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.
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E-IR-R107-50
 50mL