Vash1 (NM_177354) Mouse Tagged ORF Clone

Referência MR222520

Tamanho : 10ug

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Vash1 (NM_177354) Mouse Tagged ORF Clone

SKU
MR222520
Vash1 (Myc-DDK-tagged) - Mouse vasohibin 1 (Vash1)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

    Click here to learn more.

Bulk Requests & Clone Modifications
Specifications
Product Data
Target Symbol Vash1
Synonyms AI834978; D930046M13Rik; G630009D10Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222520 representing NM_177354
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGGGGGAAAGAAGGTGGTCCCAAGTGGCAGCAGCAGTGCCTCCCCGAACGCAGCCGCCACCACCA
CTGCTGCTGCTGCTGCTGCCGCTGCTGCCCCCCACTCTGGCACTAAACGTTTGGAGACCACCGAAGGGGC
CTCAGCACAGAGAGATGAGGAACCCGAAGAGGAAGGGGAAGAGGACCTACGAGATGGAGGTGTCCCTTTT
TTCATCAACCGAGGTGGACTGCCAGTGGATGAGGCCACCTGGGAAAGGATGTGGAAGCATGTGGCCAAGA
TCCACCCAGATGGAGAGAAGGTGGCCCTGCGCATCCGTGGGGCCACCGACCTGCCCAAGATTCCCATACC
AAGTGTGCCTACTTTCCAGCCGACGACGCCCGTCCCTGAGCGCCTGGAAGCCGTGCAGCGCTACATCAGG
GAGCTGCAGTACAATCACACAGGGACACAATTCTTTGAAATTAAGAAGAGCAGACCTCTGACGGGGCTGA
TGGACCTGGCCAAGGAAATGACCAAAGAGGCTCTGCCAATCAAATGCCTGGAAGCTGTGATCCTGGGAAT
TTACCTCACCAACAGTATGCCCACCCTGGAACGCTTTCCCATCAGCTTCAAGACCTATTTCTCAGGGAAC
TACTTCCGCCACATTGTGCTGGGGGTGAACTTCGGGGGCCGCTATGGCGCCCTGGGTATGAGCCGAAGGG
AGGACCTGATGTACAAACCGCCCGCCTTCCGCACGCTCAGTGAGCTCGTGCTGGACTACGAAGCGGCCTA
CGGCCGCTGCTGGCACGTGCTGAAGAAGGTGAAGCTGGGTCAGTGTGTGTCCCACGACCCGCACAGTGTG
GAACAGATTGAATGGAAGCACTCGGTGCTGGATGTGGAGAGGCTGGGGCGGGAAGACTTCCGCAAGGAGC
TGGAGCGTCATGCCCGAGACATGCGGCTCAAGATTGGCAAGGGGACAGGCCCTCCTTCTCCCACCAAGGA
CCGGAAGAAGGATGTTTCCTCCCCACAGAGGGCCCAGTCCAGCCCCCATCGCAGGAACAGCCGCAGTGAG
AGACGGCCTTCAGGGGAGAAGAAGCCCGCAGAGCCCAAAGCCATGCCAGACCTCAGTGGGTACCAGATCC
GGGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR222520 representing NM_177354
Red=Cloning site Green=Tags(s)

MPGGKKVVPSGSSSASPNAAATTTAAAAAAAAAPHSGTKRLETTEGASAQRDEEPEEEGEEDLRDGGVPF
FINRGGLPVDEATWERMWKHVAKIHPDGEKVALRIRGATDLPKIPIPSVPTFQPTTPVPERLEAVQRYIR
ELQYNHTGTQFFEIKKSRPLTGLMDLAKEMTKEALPIKCLEAVILGIYLTNSMPTLERFPISFKTYFSGN
YFRHIVLGVNFGGRYGALGMSRREDLMYKPPAFRTLSELVLDYEAAYGRCWHVLKKVKLGQCVSHDPHSV
EQIEWKHSVLDVERLGREDFRKELERHARDMRLKIGKGTGPPSPTKDRKKDVSSPQRAQSSPHRRNSRSE
RRPSGEKKPAEPKAMPDLSGYQIRV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_177354
ORF Size 1125 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_177354.4, NP_796328.2
RefSeq Size 6189 bp
RefSeq ORF 1128 bp
Locus ID 238328
UniProt ID Q8C1W1
Cytogenetics 12 D2
MW 42.3 kDa
Summary Tyrosine carboxypeptidase that removes the C-terminal tyrosine residue of alpha-tubulin, thereby regulating microtubule dynamics and function (PubMed:29146868). Acts as an angiogenesis inhibitor: inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis (PubMed:19204325). This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts (By similarity).[UniProtKB/Swiss-Prot Function]
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SKU Description Size
MC213050 Vash1 (untagged) - Mouse vasohibin 1 (Vash1), (10ug) 10 ug
MG222520 Vash1 (tGFP-tagged) - Mouse vasohibin 1 (Vash1), (10ug) 10 ug
MR222520L3 Lenti ORF clone of Vash1 (Myc-DDK-tagged) - Mouse vasohibin 1 (Vash1) 10 ug
MR222520L4 Lenti ORF clone of Vash1 (mGFP-tagged) - Mouse vasohibin 1 (Vash1) 10 ug
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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1830-RBPMS
 100uL