Vitellogenin (zebrafish)

Referência V2150-59H-100ul

Tamanho : 100ul

Marca : US Biological

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V2150-59H Vitellogenin (zebrafish)

Clone Type
Polyclonal
Host
mouse
Source
Zebrafish
Isotype
IgG1,k
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Ze
Shipping Temp
Blue Ice
Storage Temp
-20°C

Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.||Applications: |Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|ELISA: 1:100-1:1000. Not recommended for coating due to presence of BSA.|Western Blot: 1:100-1:1000|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: 6D845 (JE-8D6)|Host: mouse|Source: Zebrafish|Concentration: Not Determined|Form: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 12mM sodium azide|Purity: Purified by affinity chromatography|Immunogen: Vitellogenin from whole body homogenate of 17beta-estradiol treated zebrafish (Danio rerio).|Specificity: Recognizes vitellogenin in whole body homogenate samples of zebrafish (Danio rerio). Species Crossreactivity: Vitellogenin from other cyprinid species, including carp (Cyprinus carpio), fathead minnow (Pimephales promelas) and roach (Rutilus rutilus).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Vitellogenin from whole body homogenate of 17beta-estradiol treated zebrafish (Danio rerio).
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 12mM sodium azide
Purity
Purified by affinity chromatography
Specificity
Recognizes vitellogenin in whole body homogenate samples of zebrafish (Danio rerio). Species Crossreactivity: Vitellogenin from other cyprinid species, including carp (Cyprinus carpio), fathead minnow (Pimephales promelas) and roach (Rutilus rutilus).