X-Gluc (Dicyclohexylamine) [18656-96-7]
Referência HY-15935-100mg
Tamanho : 100mg
Marca : MedChemExpress
X-Gluc Dicyclohexylamine is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system).
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X-Gluc Dicyclohexylamine Chemical Structure
CAS No. : 18656-96-7
This product is a controlled substance and not for sale in your territory.
Description |
X-Gluc Dicyclohexylamine is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1]. |
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In Vitro |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Masse moléculaire |
603.93 |
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Formule |
C26H36BrClN2O7 |
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CAS No. |
18656-96-7 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
O[C@H]([C@@H](O)[C@@H]1O)[C@@H](O[C@@H]1C(O)=O)OC2=CNC3=C2C(Cl)=C(Br)C=C3.C4(CCCCC4)NC5CCCCC5 |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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Solvant et solubilité |
In Vitro:
DMSO : ≥ 30 mg/mL (49.67 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureté et documentation |
Purity: 99.48% |
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Références |
|
Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 1.6558 mL | 8.2791 mL | 16.5582 mL | 41.3955 mL |
5 mM | 0.3312 mL | 1.6558 mL | 3.3116 mL | 8.2791 mL | |
10 mM | 0.1656 mL | 0.8279 mL | 1.6558 mL | 4.1396 mL | |
15 mM | 0.1104 mL | 0.5519 mL | 1.1039 mL | 2.7597 mL | |
20 mM | 0.0828 mL | 0.4140 mL | 0.8279 mL | 2.0698 mL | |
25 mM | 0.0662 mL | 0.3312 mL | 0.6623 mL | 1.6558 mL | |
30 mM | 0.0552 mL | 0.2760 mL | 0.5519 mL | 1.3799 mL | |
40 mM | 0.0414 mL | 0.2070 mL | 0.4140 mL | 1.0349 mL |
X-Gluc Dicyclohexylamine Related Classifications
- Others
- Others
- Fluorescent Dye
-
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
X-Gluc18656-96-7Fluorescent Dyeβ-glucuronidasedye reagentE. coliGUS reporter systemmolecular biologyInhibitorinhibitorinhibit