Dilution buffers
Dilution buffers of antibodies, calibration standards and samples for immunoassays
Dilution buffers are designed to avoid interference in immunoassays that could give false positive or negative results.
If you have significant background noise or false positives, this may be due to cross-reactivity or matrix effects. It is recommended that a suitable dilution buffer be used to overcome these problems.
Antibodies, standards for calibration as well as samples such as plasma, serum, saliva, stool or food can be diluted directly with dilution buffer.
We offer a range of dilution buffers for different applications with or without tween. Indeed, it is recommended not to use tween for immunohistochemistry.
It is important to choose the right dilution buffer in order to optimize the binding between the antibodies and their targets.
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- Unconjugated 182
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- AP-staining. Click here to download PDF datasheet. Target Protein Principal Names: CD74 1
- CD74 antigen 1
- CD74 antigen (invariant polypeptide of major histocompatibility complex 1
- CD74 molecule 1
- DHLAG 1
- EBP12552 - Immunizing Peptide 1
- Goat Anti-CD74 Antibody Code Name Applications Availability Size Tested species Price Grade EB12551 Goat Anti-CD74 (aa159-171) Antibody Pep-ELISA 1
- HLA-DR-gamma 1
- HLADG 1
- IHC 100µg specific antibody in 200µl Human $324.00 Ordering Information for United States of America (change) Order direct from Everest $49.00: Overnight shipping (not applicable to SB-shifix) to United States of America Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC. Order offline OR Order from our local distributor ARP American Research Products 1
- Ia-GAMMA 1
- Ia-associated invariant chain 1
- Inc View contact details Product details Reviews Protocols and MSDS EB12551 (0.01µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence. EB12551 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer Ph 6 1
- MHC HLA-DR gamma chain 1
- NP_004346.1 Human GeneID(s): 972 Important Comments: This antibody is expected to recognize isoform a (NP_001020330.1) and isoform b (NP_004346.1). Immunogen Peptide with sequence C-KGSFPENLRHLKN 1
- NP_004346.1. Please note the peptide is available for sale. Purification and Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline 1
- Search Type in product code or name: Goat Anti-CD74 (aa159-171) Antibody (EB12551) Related Products: EBP12551 - Immunizing Peptide 1
- WB 1
- class II antigen-associated) 1
- class II invariant chain 1
- from the internal region of the protein sequence according to NP_001020330.1 1
- gamma chain of class II antigens Official Symbol: CD74 Accession Number(s): NP_001020330.1 1
- major histocompatibility complex 1
- pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. Applications Tested Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 33kDa band observed in Human Tonsil lysates (calculated MW of 33.5kDa according to NP_001020330.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml. Species Reactivity Tested: Human Expected from sequence similarity: Human 1
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Descrição
Cond.
Price Bef. VAT
NB-27-00082
1ml (4µM)
NB-27-00083
0.5ml (4µM)
GTX30933-100ml
100ml
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