HRP Chromogen for IHC - DAB
DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).
Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling
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- Unconjugated 651
- HRP 45
- Biotin 10
- APC 5
- Alexa Fluor 350 5
- Alexa Fluor 488 5
- Alexa Fluor 555 5
- Alexa Fluor 750 5
- Cy3 5
- Cy5 5
- Cy5.5 5
- Cy7 5
- FITC 5
- PE 5
- human 358
- e. coli 52
- cow 40
- camel 16
- dog 16
- hamster 16
- horse 16
- monkey 16
- pig 16
- sheep 16
- plant 5
- cat 4
- chicken 4
- donkey 4
- duck 4
- fish 4
- goose 4
- pigeon 4
- yeast 3
- virus 2
- human 241
- yeast 206
- e. coli 136
- 36
- horse 12
- virus 11
- dog 10
- hamster 10
- sheep 10
- cow 8
- monkey 8
- pig 8
- bird 5
- cat 1
- chicken 1
- donkey 1
- guinea pig 1
- Protein/peptide 1074
- Biochemicals 1034
- ELISA/ELISPOT 358
- Inhibitor/Antagonist/Agonist 269
- Primary antibody 250
- Buffers and reagents 165
- kit 103
- Other products 22
- cDNA Clone 19
- RNAi 14
- Secondary antibody 12
- Probe 6
- Lysate 2
- CRISPR/Cas9 1
- Paraffin tissue section 1
- Primer 1
- ELISA 366
- IHC 78
- WB 34
- IP 8
- 0.078 ng/mL 3
- Chemiluminescence 3
- Dot blot 3
- 0.1 ng/mL 2
- 0.116 ng/ml 1
- IF 1
- PCR 1
- Polyclonal 22
- 10.1F8 4
- 12H6.D3 4
- 13.1G11 4
- 13.2G8 4
- 13.6D9 4
- 13.7E2 4
- 13.7H4 4
- 13.8E5 4
- 13B4.E10 4
- 14.2H9 4
- 14F10.H9 4
- 1A6 4
- 26.7C4 4
- 2E4 4
- 3G8 4
- 4A8 4
- 4B9 4
- 5A11 4
- 5A2 4
- 6.1H3.D9.C6 4
- 6.1H8.F9.G6 4
- 6.2D2.E1.D11 4
- 6.7A2.A4.D3 4
- 6.8A2.B8.A11 4
- 6.8E7.A7.A1 4
- 8C7 4
- 8G1 4
- 8H12 4
- 8H6 4
- 9.21C1.G1.F1.A5 4
- 9.22E1.F5.H10 4
- 9.24E10.F3.H12 4
- IgG.2A5 4
- IgG.2E9 4
- 3
- Monoclonal 2
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