HRP Chromogen for IHC - DAB
DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).
Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling
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- Unconjugated 995
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- FITC 66
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- MaxLight 405 18
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- Cy3 14
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- PE-Cy7 1
- PerCP-Cy5.5 1
- PerCp 1
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- human 1034
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- e. coli 128
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- 48
- rat 40
- goat 36
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- dog 10
- hamster 10
- sheep 10
- virus 9
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- monkey 8
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- bird 5
- chicken 5
- cat 1
- donkey 1
- guinea pig 1
- Primary antibody 1172
- Protein/peptide 973
- Biochemicals 675
- ELISA/ELISPOT 354
- Inhibitor/Antagonist/Agonist 277
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- IP 95
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- 1G1 30
- 1G5 30
- 10i46 25
- 13H261 21
- 3H1166 21
- Monoclonal 14
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- HLy9.1.25 8
- 1A10A10 5
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- 8G1 4
- 8H12 4
- 8H6 4
- 9.21C1.G1.F1.A5 4
- 9.22E1.F5.H10 4
- 9.24E10.F3.H12 4
- IgG.2A5 4
- IgG.2E9 4
- 1626CT702.263.21 3
- 6D23 3
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- 12K274 2
- 17D17 2
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- 202.36 2
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- 2M2 2
- 3F9-2C2 2
- 5B20 2
- 7H111 2
- 8C10 2
- B2M-01 2
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- RB21227 2
- 11C257 1
- 12K99 1
- 134-2C2 1
- 13B822 1
- 5K78 1
- 5K78 (134-2C2) 1
- 6C1 1
- 6C5 1
- 9H177 1
- ADAB-8 1
- DAB-6 1
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