Phosphosense kinases are a class of enzymes that catalyze the transfer of phosphate groups to specific substrates, playing crucial roles in various metabolic pathways. These kinases are integral to cellular signaling and regulation, facilitating the phosphorylation of residues in target molecules.
These enzymes are pivotal in regulating metabolic flux, signal transduction, and the synthesis of key biomolecules. Their activity is influenced by a variety of factors, including substrate availability, energy status, and hormonal signals, reflecting their importance in maintaining cellular homeostasis.
The Phosphosense Kinases Activity Assay is a robust and sensitive tool designed to measure the enzymatic activity of phosphosense kinases in various biological samples. This assay utilizes a unique substrate specific to the kinase of interest, allowing for the detection of phosphorylated products through colorimetric or fluorescent readouts. The assay is optimized for high-throughput screening, making it suitable for both research applications and drug discovery.