Immunohistochemical staining of Giant Cell Bone Tumor (GCBT) tissue sections using Anti-Histone H3.3 G34V antibody, Clone RM307. Image courtesy of Hidetaka Yamamoto, MD, PhD, Kyushu University, Fukuoka, Japan Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding Histone H3.3 wild type or G34V mutant, stained with anti-Histone H3.3 G34V rabbit monoclonal antibody, Clone RM307. Western Blot analysis of cell lysates prepared from 293T, transfected with a DNA construct encoding wild type or G34V mutant proteins of Histone H3.3, using anti-Histone H3.3 G34V rabbit monoclonal antibody, Clone RM307.
Product Details Product Name Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody, Clone RM307 Product Description Rabbit monoclonal to Histone H3.3 G34V; Histone H3.3 G34V Mutant Catalog No. 31-1193-00-S;
31-1193-00-L;
31-1193-02 Clone Name RM307 Product Data Sheet Anti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307
Biotinylated Anti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307 Specificity RM307 reacts to the Histone H3.3 G34V mutant. No cross reactivity with wild type Histone H3.3 . Immunogen A peptide corresponding to Histone H3.3 G34V mutant Application Western Blot
ELISA
Immunohistochemistry
Species Reactivity All Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 100 µL;
400 µL;
Biotinylated 50 µL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage Western Blot: 1:1000 – 1:10000;
ELISA: 1:500 – 1:5000;
IHC: 1:100 - 1:500.
References for Histone H3.3 G34V Mutant antibody [RM307] Nambiraja A et al C19MC amplification and expression of Lin28A and Olig2 in the classification of embryonal tumors of the central nervous system: A 14-year retrospective study from a tertiary care center. Childs Nerv Syst. (2021), 10.1007/s00381-020-04973-0. IHC; Human. Read more (PubMed: 33236184)
Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602)
Sangatsuda Y et al. Base-resolution methylomes of gliomas bearing histone H3.3 mutations reveal a G34 mutant-specific signature shared with bone tumors. Neuro Oncol. (2020), 10.1093/neuonc/noaa222.836. WB; Human. Read more (PubMed: PMC7715604)
Ito Y et al. Giant cell-rich osteosarcoma of the vertebra with murine double minute chromosome 2- and cyclin-dependent kinase 4-positive and histone H3F3A mutant p.Gly34Trp-negative immunophenotypes. Pathol Int. (2018), 10.1111/pin.12650. IHC; Human. Read more (PubMed: 29446514)
Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742) . Product Images
Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody [RM307]
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Cat# 31-1193-00-S
Size : 100ul
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Rabbit monoclonal to Histone H3.3 G34V;
Histone H3.3 G34V Mutant
100 µL Small Size, 400 µL Large Size, 50 µL Biotin Conjugate
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