Reagents and instruments for immunology, cell biology and molecular biology.
 
> Aldehyde Dehydrogenase (ALDH) Activity Assay Kit

Aldehyde Dehydrogenase (ALDH) Activity Assay Kit

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Cat# E-BC-K565-M-96T

Size : 96T

Brand : Elabscience

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Phone : +1 850 650 7790

Detection Principle The detection principle of this kit is that the substrate under the action of aldehyde dehydrogenase transforms NAD+ into NADH, which under the action of electron coupling agent, transfer electrons to WST-8 to produce the yellow product. The activity of ADH can be calculated by measuring the change of absorbance value at 450 nm.
Synonyms ALDH
Sample Type Serum,Plasma,Animal tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (440-460 nm, optimum wavelength: 450 nm)
Research Area Liver And Renal Function
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.01 U/L
Detection Range 0.01-10.0 U/L
Precision inter-assay CV: 7.3% | intra-assay CV: 0.03
Assay Time 1 h 20 min
Cat.No. Product Name Sizes
E-BC-K763-M Alcohol Dehydrogenase (ADH) Activity Assay Kit 96T , 48T
E-BC-K891-M Ethanol Colorimetric Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Rat kidney tissue homogenate 4-8
10% Mouse kidney tissue homogenate 4-8
10% Mouse liver tissue homogenate 4-8
10% Mouse lung tissue homogenate 4-8
Mouse serum 4-8
Rat serum 4-8
Human serum 4-8
Rat plasma 4-8

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

Other Clones

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Other Formats

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor®405

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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