Western Blot analysis of cell lysates prepared from 293T, transfected with a DNA construct encoding G34R mutant or wild type proteins of Histone H3.3, using anti-Histone H3.3 G34R, Clone RM240. IHC of clinically validated FFPE Glioblastoma tumor tissues with H3.3 G34R expression, using anti-histone H3.3 G34R rabbit monoclonal antibody, Clone RM240. Clinically validated to be Positive for H3.3 G34R Mutation. Image courtesy of Dr. Sebastian Brandner, UCL Institute of Neurology, London, United Kingdom IHC of clinically validated FFPE Glioblastoma tumor tissues, using anti-histone H3.3 G34R rabbit monoclonal antibody, Clone RM240. Clinically validatedto be Negative for H3.3 G34R Mutation. Image courtesy of Dr. Sebastian Brandner, UCLInstitute of Neurology, London, United Kingdom ICC staining of primary human cell lines expressing WT and H3.3 G34R Mutation. Image courtesy of Dr. Nada Jabado, McGill University, Montreal, Quebec, Canada.
Product Details Product Name Anti-Histone H3.3 G34R Rabbit Monoclonal Antibody, Clone RM240 Product Description Rabbit monoclonal to Histone H3.3 G34R; Histone H3.3 G34R Mutant Catalog No. 31-1120-00-S;
31-1120-00-L;
31-1120-02 Clone Name RM240 Product Data Sheet Anti-Histone H3.3 G34R Mutant Rabbit Monoclonal Antibody Data Sheet RM240
Biotinylated Anti-Histone H3.3 G34R Mutant Rabbit Monoclonal Antibody Data Sheet RM240 Specificity RM240 reacts to the Histone H3.3 G34R mutant. No cross reactivity with wild type Histone H3.3 . Immunogen A peptide corresponding to Histone H3.3 G34R mutant Application Western Blot
ELISA
Immunohistochemistry
Chromatin IP Species Reactivity All Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 100 µL;
400 µL;
Biotinylated 50 µL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage Western Blot: 1:200 – 1:1000;
ELISA: 1:250 – 1:2500;
IHC: 1:100 – 1:500. References for Histone H3.3 G34R Mutant antibody [RM240] Nambiraja A et al C19MC amplification and expression of Lin28A and Olig2 in the classification of embryonal tumors of the central nervous system: A 14-year retrospective study from a tertiary care center. Childs Nerv Syst. (2021), 10.1007/s00381-020-04973-0. IHC; Human. Read more (PubMed: 33236184)
Esteve-Codina A et al RNA sequencing and Immunohistochemistry Reveal ZFN7 as a Stronger Marker of Survival than Molecular Subtypes in G-CIMP-negative Glioblastoma. Clin Cancer Res. (2021), 10.1158/1078-0432.CCR-20-2141. IHC; Human. Read more (PubMed: 33106291)
Bressa RB et al Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutants. Cell Stem Cell. (2021), 10.1016/j.stem.2021.01.016. IHC; Human. Read more (PubMed: 33631116)
Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602)
Pathania M et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017) 10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533)
Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742) . Product Images
Anti-H3.3G34R Mutant rabbit monoclonal antibody [RM240]
![Anti-H3.3G34R Mutant rabbit monoclonal antibody [RM240]](https://www.quimigen.pt/en/upload/-img-lapin1.png)
Cat# 31-1120-00-S
Size : 100ul
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Rabbit monoclonal to Histone H3.3 G34R;
Histone H3.3 G34R Mutant
100 µL Small Size, 400 µL Large Size, 50 µL Biotin Conjugate
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