Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
Tested applications:
ELISA (ELISA), Immunoflourescence (IF)
Recommended dilution:
1:10 (ELISA, IF)
Reactivity
Confirmed reactivity:
Higher plants, ferns and mosses
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Has no known cross-reactivity with other polymers. Binds to unesterified homogalacturonan.
The antibody recognizes a range of homogalacturonan samples but binds strongly to un-esterified homogalacturonan.
Background
Background:
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.
Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.
Product Citations
Selected references:
Lima et al. (2024). Seed coat-derived brassinosteroid signaling regulates endosperm development. Nat Commun. 2024 Oct 29;15(1):9352. doi: 10.1038/s41467-024-53671-x. Pan, Li, Liu, Qi et al. (2023) Multi-microscopy techniques combined with FT-IR spectroscopy reveals the histological and biochemical causes leading to fruit texture difference in oriental melon (Cucumis melo var. Makuwa Makino), Food Chemistry,Volume 402,´2023,134229, ISSN 0308-8146, https://doi.org/10.1016/j.foodchem.2022.134229.(https://www.sciencedirect.com/science/article/pii/S0308814622021914) Marcus et al. (2010). Restricted access of proteins to mannan polysaccharides in intact plant cell walls. Plant J. 2010 Oct;64(2):191-203.doi: 0.1111/j.1365-313X.2010.04319.x. Verhertbruggen et al. (2009). An extended set of monoclonal antibodies to pectic homogalacturonan. Carbohydr Res. 2009 Sep 28;344(14):1858-62.doi: 10.1016/j.carres.2008.11.010.
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