Aspartate Aminotransferase (AST/GOT) Activity Assay Kit

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Cat# E-BC-K236-M-500

Size : 500Assays

Contact local distributor :

Phone : +1 850 650 7790

Brand : Elabscience

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Contact local distributor :

Phone : +1 850 650 7790

Detection Principle	AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
Synonyms	AST;GOT;Glutamic-oxalacetic transaminase
Sample Type	Serum,Plasma,Animal tissue,Cell,Cell culture supernatant
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (500-520 nm)
Research Area	Liver And Renal Function
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.10 IU/L
Detection Range	1.10-72.30 IU/L
Precision	inter-assay CV: 6.8% \| intra-assay CV: 5.3%
Assay Time	1 h 20 min

Cat.No.	Product Name	Sizes
E-BC-K235-M	Alanine Aminotransferase (ALT/GPT) Activity Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Human plasma	1
Porcine serum	1
Rat serum	1
HC-60 cellular supernatant	1
Calu-3 cellular supernatant	1
10% Rat liver tissue homogenization	15-30
10% Rat lung tissue homogenization	2-8

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.