BCIP/NBT One Component Membrane Alkaline Phosphatase (AP) Substrate (Blue)

Store at temperatures from 225ºC. Do Not Freeze

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Stable for a minimum of 3 years from the manufactured date when stored between 225ºC. The substrate should not be frozen and should be protected from direct light by storing in amber bottles. Only high quality amber glass and plastic products should be used for storing aliquots.

This BCIP/NBT One component buffered alkaline phosphatase substrate (5bromo4chloro3indolylphosphate and nitroblue tetrazolium) is a precipitating substrate used with the enzyme alkaline phosphatase designed for various membrane immunoassays but not recommended for microwell or immunohistochemical applications. Initially, the unreacted substrate should be colorless to light yellow in appearance. When this substrate system is reacted with alkaline phosphatase, an insoluble, permanent dark blue reaction product is obtained.

Leinco Technologies’ BCIP/NBT One Component Membrane Substrate is a highly sensitive, high performance formulation that produces a high signal to noise ratio with low membrane background. The outstanding shelf life of at least thirty six months for the BCIP/NBT One Component Membrane Substrate makes this reagent ideal for long term use of the same manufacturing lot.

BCIP/NBT One Component Membrane Substrate is a ready to use solution that needs no preparation or dilution. This product should be at room temperature (25°C) before use. Pour the necessary amount of substrate onto the blocked alkaline phosphatase conjugate labeled membrane to completely cover the membrane. The substrate will react with sites on the membrane containing phosphatase, producing an insoluble blue reaction product. The reaction should be monitored and read before the background color becomes too intense which may result in diminished contrast between the positive and background staining. To stop the reaction, rinse membrane with reagent quality water. Some wash solutions containing detergents may reduce color intensities. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.