Sandwich ELISA using RM121 as the capture antibody Sandwich ELISA using RM121 as the capture antibody ELISA of human immunoglobulins shows RM121 A titer ELISA using RM121. The plate was coated
Product Details Product Name Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121 Product Description Biotinylated Rabbit monoclonal to Human IgM Catalog No. 31-1023-02 Clone Name RM121 Product Data Sheet Biotin Anti-Human IgM Rabbit Monoclonal Antibody Data Sheet RM121 Specificity RM121 reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE. Immunogen Human IgM Application ELISA
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Species Reactivity Human Conjugate Biotin Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 50 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL. Keywords . Product Images
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
reacts only to human IgM. No cross reactivity
with Human IgG, IgE, IgD, or IgA. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL of RM121 was used as
the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.
with different amounts of human IgM. A serial dilution
of RM121 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
Biotin Anti-Human IgM rabbit monoclonal antibody [RM121]
Cat# 31-1023-02
Size : 50ug
Brand : RevMab Biosciences
Biotinylated Rabbit monoclonal to Human IgM
50 µg