Calcium (Ca) Colorimetric Assay Kit

Cat# E-BC-K103-M-96T

Size : 96T

Brand : Elabscience

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Phone : +1 850 650 7790

Detection Principle Calcium ion in the sample bind to Methyl Thymol Blue (MTB) in alkaline solution and form blue complex. The blue complex has a specific absorption peak at 715 nm and calcium content can be calculated by measuring the OD value at 610 nm.
Synonyms Ca
Sample Type Serum,Plasma,Urine,Tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (600-620 nm, optimum wavelength: 610 nm)
Research Area Plant Physiology Series ,  Inorganic Ions
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.07 mmol/L
Detection Range 0.07-1.2 mmol/L
Precision inter-assay CV: 8.5% | intra-assay CV: 4.7%
Assay Time 20 min
Cat.No. Product Name Sizes
E-BC-K137-M Zinc (Zn) Colorimetric Assay Kit 96T , 48T
E-BC-K139-M Iron Colorimetric Assay Kit 96T , 48T
E-BC-K162-M Magnesium (Mg) Colorimetric Assay Kit 96T , 48T
E-BC-K245-M Phosphorus (Pi) Colorimetric Assay Kit (Phospho Molybdate Method) 96T , 48T
E-BC-K279-M Potassium (K) turbidimetric Assay Kit 96T , 48T
E-BC-K300-M Copper (Cu2+) Colorimetric Assay Kit (Complexing Method) 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Dog serum 2-3
Human serum 3-6
Mouse serum 3-6
Human urine 4-8
20% Animal tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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