Citrate Synthase(CS) Activity Assay Kit

Cat# E-BC-K178-M-48T

Size : 48T

Brand : Elabscience

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Detection Principle Citrate synthetase (CS) catalyzes acetyl-CoA and oxaloacetate to produce limonyl-CoA, which can further produce CoA. The yellow product generated by this reaction has a characteristic absorption peak at 412 nm. The activity of CS can be calculated by measuring the change of absorbance value at 412 nm.
Synonyms CS
Sample Type Animal tissue,Plant tissue,Cell
Detection Method Colorimetric method
Detection Instrument Microplate reader (402-422 nm, optimum wavelength: 412 nm)
Research Area Mitochondria Function ,  Tricarboxylic Acid(TCA)Cycle ,  Energy Metabolism
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 2.09 U/L
Detection Range 2.09-110 U/L
Precision inter-assay CV: 7.9% | intra-assay CV: 0.05
Assay Time 30 min
Cat.No. Product Name Sizes
E-BC-K351-M Citric Acid (CA) Colorimetric Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Rat kidney tissue homogenate 1
10% Rat spleen tissue homogenate 1
10% Rat liver tissue homogenate 1
10% Rat heart tissue homogenate 1
10% Mouse liver tissue homogenate 1
10% Mouse lung tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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Description
Cond.
Price Bef. VAT
E-BC-K178-M-96T
 96T