EasyPure® RNA Kit
Cat# ER101-01
Size : 50rxns
Brand : TransGen Biotech
Contact local distributor :
Phone : +1 850 650 7790
ManualEasyPure® RNA Kit provides a simple and fast column based method to isolate total RNA from animal cells, animal tissues, bacteria and yeast. Cells and tissues are enzymatically lysed. DNA is digested with DNase I. RNA is bound to silica membrane. After washing, high quality RNA is eluted from the column. RNA is free of protein contamination, and is suitable for RT-PCR, qRT-PCR and Northern blot.
Storage
DNase I and DNase I Reaction Buffer at -20°C for one year; others at room temperature (15°C-25°C) in a dry place for one year.
Shipping
DNase I and DNase I Reaction Buffer on dry ice (-70 °C); Others at room temperature.
| Component | ER101-01 (50 rxns) |
| Binding buffer 4 (BB4) | 40 ml |
| Clean buffer 4 (CB4) | 60 ml |
| Wash buffer 4 (WB4) | 12 ml |
| Proteinase K (20 mg/ml) | 1 ml |
| DNase I (3 units/μl) | 1500 units |
| DNase I Reaction Buffer | 4×1 ml |
| RNase-free Water | 10 ml |
| RNase-free Tube (1.5 ml) | 50 each |
| RNA Spin Columns with Colle ction Tub e s | 50 each |
Han X , Wang R , Zhou Y , et al. Mapping the Mouse Cell Atlas by Microwell-Seq[J]. Cell, 2018, 172(5):1091-1107.
Li P, Du J, Goodier JL, et al. Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion[J]. Nucleic Acids Research, 2017 May 5;45(8):4619-4631.
Simultaneous NF-κB inhibition and E-cadherin uPregulation mediate mutually synergistic anticancer activity of celastrol and SAHA in vitro and in vivo[J].International Journal of Cancer, 2014, 135(7):1721-32.
Tan G , Xiao Q , Song H , et al. Type I IFN augments IL-27-dependent TRIM25 expression to inhibit HBV replication[J]. Cellular & Molecular Immunology, 2018, 15(3):272-281.
Zheng K , Tian H , Hu Q , et al. Ectopic expression of R3 MYB transcription factor gene OsTCL1 in Arabidopsis, but not rice, affects trichome and root hair formation[J]. Scientific Reports, 2016, 6(1):19254.