Fab Anti-RABBIT IgG (H&L) (DONKEY) Antibody

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Cat# 811-7102

Size : 1mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : Rockland Immunochemicals

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Contact local distributor :

Phone : +1 850 650 7790

Specifications for Fab Rabbit IgG (H&L) Antibody

Product Details

Description: Donkey Fab Anti-Rabbit IgG (H&L) Antibody - 811-7102

Synonyms: Donkey Fab Anti-Rabbit IgG Antibody, Donkey Fab Fragment Anti-Rabbit IgG Antibody

Host Species: Donkey

Specificity: IgG (H&L)

Clonality: Polyclonal

Format: IgG Fab

Target Details

Reactivity: Rabbit

Immunogen: Rabbit IgG whole molecule

Purity/Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).

Application Details

Suggested Applications: IHC - View References

Application Note: Fab Anti-Rabbit IgG (H&L) Antibody is suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Formulation

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Fab Anti-Rabbit IgG (H&L) Antibody generated in donkey detects immunoglobulin g from rabbit, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

References (1)

Eckstein E et al. (2020). Cyclic regulation of Trpm4 expression in female vomeronasal neurons driven by ovarian sex hormones. Mol Cell Neurosci.

Applications

IHC, ICC, Histology

PubMed

Related Protocols

Heat-induced Antigen Retrieval Protocol

Immunohistochemistry (IHC) Protocol

Protease-induced Antigen Retrieval Protocol

Staining Paraffin Sections by PAP Procedure

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.