Goat Anti-Mouse IgG2a, Human ads-PE
Cat# 1080-09
Size : 0.5mg
Brand : Southern Biotech
Contact local distributor :
Phone : +1 850 650 7790
| Isotype | Goat IgG |
|---|---|
| Isotype Control | Goat IgG-PE |
| Specificity | Reacts with the heavy chain of mouse IgG2a |
| Source | Pooled antisera from goats hyperimmunized with mouse IgG2a |
| Cross Adsorption | Mouse IgG1, IgG2b, IgG3, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species |
| Purification Method | Affinity chromatography on mouse IgG2a covalently linked to agarose |
| Conjugate | PE (R-phycoerythrin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/mL |
| Volume | 0.5 mL or 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
| Applications | Quality tested applications for relevant formats include - ELISA 1-9 FLISA Flow Cytometry 11-13 Other referenced applications for relevant formats include - ELISpot 3,10 Immunohistochemistry-Frozen Sections 2,14,15 Immunohistochemistry-Paraffin Sections 16,17 Immunocytochemistry 4,18-20 Electron Microscopy 14 Western Blot 1,21-23 Surface Plasmon Resonance 24 Purification 25 |
| RRID Number | AB_2794481 |
| Gene ID | 380793 (Mouse) |
| Gene ID Symbol | Ighg2a (Mouse) |
| Gene ID Aliases | 1810060O09Rik; Igh-1; Igh-1a |
| UniProt ID | P01863 (Mouse P01864 (Mouse P01865 (Mouse |
| UniProt Name | GCAA_MOUSE (Mouse) GCAB_MOUSE (Mouse) GCAM_MOUSE (Mouse) |
Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup
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- 1. Martin RM, Brady JL, Lew AM. The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice. J Immunol Methods. 1998;212:187-92. (ELISA, WB)
- 2. Richards HB, Satoh M, Jennette JC, Croker BP, Yoshida H, Reeves WH. Interferon-γ is required for lupus nephritis in mice treated with the hydrocarbon oil pristane. Kidney Int. 2001;60:2173-80. (ELISA, IHC-FS)
- 3. Cox RJ, Mykkeltvedt E, Robertson J, Haaheim LR. Non-lethal viral challenge of influenza haemagglutinin and nucleoprotein DNA vaccinated mice results in reduced viral replication. Scand J Immunol. 2002;55:14-23. (ELISA, ELISPOT)
- 4. Swiecki MK, Lisanby MW, Shu F, Turnbough CL Jr, Kearney JF. Monoclonal antibodies for Bacillus anthracis spore detection and functional analyses of spore germination and outgrowth. J Immunol. 2006;176:6076-84. (ELISA, ICC)
- 5. Arnaboldi PM, Roth-Walter F, Mayer L. Suppression of Th1 and Th17, but not Th2, responses in a CD8+ T cell mediated model of oral tolerance. Mucosal Immunol. 2009;2:427-38. (ELISA)
- 6. Faustino L, Fonseca DM, Florsheim EB, Resende RR, Lepique AP, Faquim-Mauro E, et al. Tumor necrosis factor-related apoptosis-inducing ligand mediates the resolution of allergic airway inflammation induced by chronic allergen inhalation. Mucosal Immunol. 2014;7:1199-208. (ELISA)
- 7. Hartung E, Becker M, Bachem A, Reeg N, Jäkel A, Hutloff A, et al. Induction of potent CD8 T cell cytotoxicity by specific targeting of antigen to cross-presenting dendritic cells in vivo via murine or human XCR1. J Immunol. 2015;194:1069-79. (ELISA)
- 8. Dabral N, Jain-Gupta N, Seleem MN, Sriranganathan N, Vemulapalli R. Overexpression of Brucella putative glycosyltransferase WbkA in B. abortus RB51 leads to production of exopolysaccharide. Front Cell Infect Microbiol. 2015;5:54. (ELISA)
- 9. Dabaghian M, Latifi AM, Tebianian M, Dabaghian F, Ebrahimi SM. A truncated C-terminal fragment of Mycobacterium tuberculosis HSP70 enhances cell-mediated immune response and longevity of the total IgG to influenza A virus M2e protein in mice. Antiviral Res. 2015;120:23-31. (ELISA)
- 10. Teichmann LL, Schenten D, Medzhitov R, Kashgarian M, Shlomchik MJ. Signals via the adaptor MyD88 in B cells and DCs make distinct and synergistic contributions to immune activation and tissue damage in lupus. Immunity. 2013;38:528-40. (ELISPOT)
- 11. Davis ME, Maxwell CV, Erf GF, Brown DC, Wistuba TJ. Dietary supplementation with phosphorylated mannans improves growth response and modulates immune function of weanling pigs. J Anim Sci. 2004;82:1882-91. (FC)
- 12. Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, et al. Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β. Nature. 2012;484:514-8. (FC)
- 13. Sutton KM, Hu T, Wu Z, Siklodi B, Vervelde L, Kaiser P. The functions of the avian receptor activator of NF-κB ligand (RANKL) and its receptors, RANK and osteoprotegerin, are evolutionarily conserved. Dev Comp Immunol. 2015;51:170-84. (FC)
- 14. Raats CJ, van den Born J, Bakker MA, Oppers-Walgreen B, Pisa BJ, Dijkman HB, et al. Expression of agrin, dystroglycan, and utrophin in normal renal tissue and in experimental glomerulopathies. Am J Pathol. 2000;156:1749-65. (IHC-FS, EM)
- 15. Poosti F, Bansal R, Yazdani S, Prakash J, Post E, Klok P, et al. Selective delivery of IFN-γ to renal interstitial myofibroblasts: a novel strategy for the treatment of renal fibrosis. FASEB J. 2015;29:1029-42. (IHC-FS)
- 16. Huizinga R, van der Star BJ, Kipp M, Jong R, Gerritsen W, Clarner T, et al. Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis. Glia. 2012;60:422-31. (IHC-PS)
- 17. Kreijtz JH, Wiersma LC, De Gruyter HL, Vogelzang-van Trierum SE, van Amerongen G, Stittelaar KJ, et al. A single immunization with modified vaccinia virus Ankara-based influenza virus H7 vaccine affords protection in the influenza A(H7N9) pneumonia ferret model. J Infect Dis. 2015;211:791-800. (IHC-PS)
- 18. Albrecht B, Woisetschläger M, Robertson MW. Export of the high affinity IgE receptor from the endoplasmic reticulum depends on a glycosylation-mediated quality control mechanism. J Immunol. 2000;165:5686-94. (ICC)
- 19. Häggqvist B, Hultman P. Effects of deviating the Th2-response in murine mercury-induced autoimmunity towards a Th1-response. Clin Exp Immunol. 2003;134:202-9. (ICC)
- 20. Mia MM, Bank RA. Paracrine factors of human amniotic fluid-derived mesenchymal stem cells show strong anti-fibrotic properties by inhibiting myofibroblast differentiation and collagen synthesis. J Stem Cell Res Ther. 2015;5:5. (ICC)
- 21. Santoro L, Reboul A, Kerblat I, Drouet C, Colomb MG. Monoclonal IgG as antigens: reduction is an early intracellular event of their processing by antigen-presenting cells. Int Immunol. 1996;8:211-9. (WB)
- 22. Attramadal CG, Halstensen TS, Dhakal HP, Ulekleiv CH, Boysen ME, Nesland JM, et al. High nuclear SOX2 expression is associated with radiotherapy response in small (T1/T2) oral squamous cell carcinoma. J Oral Pathol Med. 2015;44:515-22. (WB)
- 23. Konitsiotis AD, Jovanović B, Ciepla P, Spitaler M, Lanyon-Hogg T, Tate EW, et al. Topological analysis of Hedgehog acyltransferase, a multipalmitoylated transmembrane protein. J Biol Chem. 2015;290:3293-307. (WB)
- 24. Sun Y, Peng I, Senger K, Hamidzadeh K, Reichelt M, Baca M, et al. Critical role of activation induced cytidine deaminase in experimental autoimmune encephalomyelitis. Autoimmunity. 2013;46:157-67. (Surface Plasmon Resonance)
- 25. Chmielewski M, Hombach AA, Abken H. CD28 cosignalling does not affect the activation threshold in a chimeric antigen receptor-redirected T-cell attack. Gene Ther. 2011;18:62-72. (Purification)