Guinea Pig Polyclonal Anti-Keratin K10
Cat# GP-K10
Size : 100ul
Brand : Progen
Contact local distributor :
Phone : +1 850 650 7790
| Quantity | 100 µl |
|---|---|
| Antibody Type | Polyclonal |
| Host | Guinea pig |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Stabilized antiserum |
| Reactivity | Human, Mouse, Rat |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Synthetic peptide of human keratin K10 (formerly also designated cytokeratin 10; C-GS VGE SSS KGP RY), coupled to KLH |
| Formulation | Contains 0.09% sodium azide and 0.5% BSA |
| UniprotID | P13645 (Human),P02535 (Mouse),Q6IFW6 (Rat) |
| Synonym | Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP |
| Note | Centrifuge prior to opening |
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:100-1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:100-1:200 (microwave treatment recommended) |
| Western Blot (WB) | 1:250-1:1,000 |
MW 56,5000 (pI 5.3) intermediate filament polypeptide, keratin K10, detected by immunohistochemistry in the suprabasal layers of human epidermis (basal cells are negative). Useful for the recognition of keratinizing cells in squamous cell carcinoma of epidermis, lung, bladder, cervix, esophagus etc. Completely negative on non-stratified epithelia.
Positive control: human suprabasal keratinocytes of epidermis; squamous cell carcinoma.
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