MDM2 antibody
Cat# GTX100531-100ul
Size : 100ul
Brand : GeneTex
Host | Rabbit |
---|---|
Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IP, ChIP assay |
Reactivity | Human, Mouse |
Package | 100 μl, 25 μl |
APPLICATION
Application Note
Application | Recommended Dilution |
---|---|
WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IP | 1:100-1:500 |
ChIP assay | Assay dependent |
Calculated MW
Positive Control
Predict Reactivity
PROPERTIES
Form
Buffer
Preservative
Storage
Concentration
Antigen Species
Immunogen
Purification
Conjugation
RRID
Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Cellular Localization
Background
Database
DATA IMAGES
GTX100531 IHC-P ImageMDM2 antibody detects MDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.MDM2 stained by MDM2 antibody (GTX100531) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100531 IHC-P Image Immunohistochemical analysis of paraffin-embedded human lung cancer, using MDM2(GTX100531) antibody at 1:500 dilution. |
GTX100531 IP Image MDM2 antibody immunoprecipitates MDM2 protein in IP experiments. |
GTX100531 WB ImageVarious whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100531 IHC-P ImageMDM2 antibody detects MDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.MDM2 stained by MDM2 antibody (GTX100531) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX100531 ChIP assay Image Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of either control rabbit IgG or anti-MDM2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter. |
GTX100531 WB ImageUntreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX100531 ICC/IF Image MDM2 antibody detects MDM2 protein at cytoplasm and nucleus by immunofluorescent analysis. |
GTX100531 WB ImageUntreated (–) and treated (+) HCT116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
Application Reference
REVIEW
MDM2 antibody Cat. No. GTX100531
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Western Blot(WB) | | ( Average 5 based on 1 users reviews) |