Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method
Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration
1.0 mg/mL - lot specific
Conjugation
Biotin
Storage
Store second antibody at 4 secondary antibody is available in a variety of formats. Anti-Mouse IgG3 secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG3 antibody based assays.
