Mouse IgM Isotype Control
Cat# AM03111FC-N
Size : 100ug
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Mouse IgM Isotype Control
| Product Data | |
| Application | FC |
|---|---|
| Antibody Host | Mouse |
| Isotype | IgM |
| Clonality | Monoclonal |
| Specificity | This antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen. |
| Buffer | Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 |
| Concentration | 1 mg/ml |
| Conjugation | FITC |
| Storage | Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf life: one year from despatch. |
| Background | The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PFR-03, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgM molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. |