Normal Rat Serum

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Size : 10mLx10

Phone : +1 850 650 7790

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Phone : +1 850 650 7790

Product name	Normal Rat Serum
Host	Rat
Applications	BL, ELISA, ICC, IF, IHC
Applications notes	Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-p (1:10-20), IF/ICC (1:10-20) and ELISA (1:100).
Preparation method	Normal rat serum is processed from blood collected from non-immunized normal adult rats. Hemolysis is minimized by employing proprietary techniques during the blood collection and serum extraction processes.
Preparation method	Lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2
Purity	Whole serum

Formulation

Liquid solution

Storage buffer

10 mM sodium phosphate, 0.15 M sodium chloride with 0.05% sodium azide as preservative, pH 7.2

Storage instructions

Store at 2-8 class="pro-form-title">Additional Information

Background	Normal rat serums, which are from non-immuned rat hosts, are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide. Normal serum diluted to 5%-20% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific binding, or control for most immunoassay applications. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.

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